Abstract: Objective To detect the expression levels of microRNA-193a-3p(miR-193a-3p) and inhibitor of growth gene 1(ING1) in esophageal cancer tissue,and to explore the relationship between them and clinicopathological parameters and prognosis.
Methods The esophageal cancer and adjacent paracancerous tissues were collected from 90 patients with esophageal cancer who were treated in our hospital from January 2013 to June 2014.Quantitative real-time PCR(qRT-PCR) was used to detect miR-193a-3p expression in esophageal cancer and adjacent paracancerous tissues.Immunohistochemistry(IHC) was used to detect ING1 expression in esophageal cancer and adjacent paracancerous tissues.Target Scan7.1 was used to predict and analyze targeting relationship of miR-193a-3p and ING1.Spearman rank correlation analysis was used to detecte the correlation between miR-193a-3p and ING1 expression in esophageal cancer tissues.The relationship between the expression of miR-193a-3p,ING1 and the clinicopathological parameters and prognosis of esophageal cancer were analyzed.
Results The expression of miR-193a-3p in esophageal cancer tissues was significantly higher than that in adjacent paracancerous tissues(P<0.05),and the positive expression rate of ING1 in esophageal cancer tissues was lower than that in adjacent paracancerous tissues(P<0.05).Target scan 7.1 predicted that there was a binding site of miR-193a-3p in 3'UTR region of ING1.There was a negative correlation between the expression of miR-193a-3p and ING1 in esophageal cancer tissues(P<0.05).The expression of miR-193a-3p and ING1 in esophageal cancer was related to lymph node metastasis and TNM stage(P<0.05).Kaplan Meier survival curve showed that the survival rate of esophageal cancer patients with high expression of miR-193a-3p was lower than that of esophageal cancer patients with low expression of miR-193a-3p(P<0.05),and the survival rate of esophageal cancer patients with negative expression of ING1 was lower than that of esophageal cancer patients with positive expression of ING1(P<0.05).
Conclusion The expressions of miR-193a-3p and ING1 are abnormal in esophageal cancer,and the expressions of miR-193a-3p and ING1 are related to the poor pathological parameters and prognosis of esophageal cancer.Mir-193a-3p may promote the development of esophageal cancer by regulating ING1.

Key words: esophageal cancer, MiR-193a-3p, inhibitor of Growth Gene 1, clinicopathological parameters