Abstract: Objective To investigate the expression of N-acetyltransferase 10 (NAT10),serine hydroxymethyltransferase 2 (SHMT2) and YTH domain family protein 1 (YTHDF1) in lung cancer tissues and their correlation with clinicopathological characteristics and prognosis. Methods A total of 98 lung cancer patients admitted to our hospital from April 2020 to April 2021 were regarded as the observation subjects.The cancerous tissues and adjacent tissues of the patient during surgery were collected.Immunohistochemistry was used to detect the expression levels of NAT10,SHMT2 and YTHDF1.The patients were followed up for 3 years and divided into the survival group and the death group according to their prognosis.The data of general clinicopathological characteristics were collected and analyzed.The relationship between NAT10,SHMT2 and YTHDF1 with the prognosis of patients were analyzed.Multivariate Cox- regression was used to analyze the influencing factors of lung cancer patients.Results The high expression ratios of NAT10,SHMT2 and YTHDF1 in cancer tissues were obviously higher than those in adjacent tissues (P<0.05).The expression of NAT10 and YTHDF1 was related to clinical stage,degree of differentiation and lymph node metastasis (P<0.05),and the expression of SHMT2 was correlated with clinical stage,degree of differentiation,tumor diameter,and lymph node metastasis (P<0.05).There were statistically significant differences in tumor diameter, clinical stage, degree of differentiation, lymph node metastasis, and expressions of NAT10, SHMT2, and YTHDF1 between the survival group and the death group (P<0.05). NAT10, SHMT2, YTHDF1 patient survival rates significantly below the low of high expression patients (χ2=6.354, P=0.012, χ 2 =8.512, P=0.004, χ2=4.791, P=0.029).Lymph node metastasis, high expression of NAT10, SHMT2 and YTHDF1 are all risk factors affecting the prognosis of patients (P < 0.05 ).Conclusion NAT10,SHMT2,and YTHDF1 are all highly expressed in the tissues of lung cancer,and have a certain correlation with clinical pathological characteristics and prognosis.They may serve as relevant evaluation indicators for the prognosis of lung cancer patients.

Key words: N-acetyltransferase 10, serine hydroxymethyltransferase 2, YTH domain family protein 1, lung cancer, clinical staging, prognosis