临床外科杂志 ›› 2023, Vol. 31 ›› Issue (12): 1144-1147.doi: 10.3969/j.issn.1005-6483.2023.12.010

• 论著 • 上一篇    下一篇

长链非编码RNA  NORAD和微小-RNA-199a-3p在食管鳞癌病人血清中的表达及其临床意义

  

  1. 223800 江苏省宿迁市第一人民医院胸心外科 
  • 收稿日期:2023-02-24 出版日期:2023-12-20 发布日期:2023-01-15
  • 通讯作者: 臧其威，Email:jssqzqw@163.com
  • 基金资助:
    江苏省宿迁市科技计划项目(Y0204)

Expression and clinical significance of long non-coding RNA NORAD and miR-199a-3p in serum of patients with esophageal squamous cell carcinoma

  1. Department of Thoracic and Cardiac Surgery,Suqian First People’s Hospital,Jiangsu,Suqian 223800, China
  • Received:2023-02-24 Online:2023-12-20 Published:2023-01-15

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摘要： 目的 探讨长链非编码RNA NORAD(LncRNA NORAD)、微小RNA-199a-3p(miR-199a-3p)在食管鳞癌病人血清中的表达，分析其对食管鳞癌的诊断价值。方法 2018年1月~2022年12月间我院收治的食管鳞癌病人286例（食管鳞癌组），记录其临床病理特征，同期纳入健康体检者204例（对照组）。采用实时荧光定量PCR(qRT-PCR)法检测血清LncRNA NORAD、miR-199a-3p表达水平；采用Pearson相关性分析血清LncRNA NORAD与miR-199a-3p表达水平的相关性；采用受试者工作特征(ROC)曲线分析血清LncRNA NORAD、miR-199a-3p表达水平对食管鳞癌的诊断价值。结果 食管鳞癌组血清LncRNA NORAD表达水平高于对照组，miR-199a-3p表达水平低于对照组，差异有统计学意义(P<0.05)。食管鳞癌病人血清LncRNA NORAD与miR-199a-3p表达水平呈负相关(r=-0.649，P<0.05)。有淋巴结转移、TNM分期为T3~T4期、低分化的食管鳞癌病人LncRNA NORAD高表达、miR-199a-3p低表达比例高于无淋巴结转移、TNM分期为T1~T2期、中/高分化的食管鳞癌病人，差异有统计学意义(P<0.05)。LncRNA NORAD、miR-199a-3p单独及二者联合诊断食管鳞癌的AUC分别为0.817、0.796、0.854。结论 食管鳞癌病人血清LncRNA NORAD高表达，miR-199a-3p低表达，二者表达水平可作为食管鳞癌的辅助诊断指标之一。

关键词: 食管鳞癌, 血清, 长链非编码RNA NORAD, 微小RNA-199a-3p

Abstract: Objective To investigate the difference in the expression of long non-coding RNA NORAD(LncRNA NORAD) and microRNA-199a-3p(miR-199a-3p) in the serum of patients with esophageal squamous cell carcinoma and healthy people,and analyze their diagnostic value for esophageal squamous cell carcinoma. Methods A total of 286 patients with esophageal squamous cell carcinoma admitted to our hospital from January 2018 to December 22 were included in the esophageal squamous cell carcinoma group,the clinicopathological characteristics were recorded,in the same period,204 healthy people excluding esophageal squamous cell carcinoma and other systemic diseases were included as the healthy control group.The expression levels of serum LncRNA NORAD and miR-199a-3p were detected by real-time fluorescence quantitative PCR(qRT-PCR);Pearson correlation was applied to analyze the correlation between serum LncRNA NORAD and miR-199a-3p expression levels;the diagnostic value of serum LncRNA NORAD and miR-199a-3p expression levels in esophageal squamous cell carcinoma was analyzed by the ROC curve. Results The expression level of serum LncRNA NORAD in esophageal squamous cell carcinoma group was higher than that in healthy control group,the expression level of miR-199a-3p was obviously lower than that in the healthy control group(P<0.05).There was a negative correlation between serum LncRNA NORAD and miR-199a-3p expression in patients with esophageal squamous cell carcinoma(r=-0.649,P<0.05).The proportions of high expression of LncRNA NORAD and low expression of miR-199a-3p in patients with lymph node metastasis,TNM stage T3+T4 and poorly differentiated esophageal squamous cell carcinoma were higher than those in patients with no lymph node metastasis,TNM stage T1+T2 and moderately/highly differentiated esophageal squamous cell carcinoma,with statistically obvious difference(P<0.05).The AUC of LncRNA NORAD and miR-199a-3p in the diagnosis of esophageal squamous cell carcinoma was 0.817,0.796 and 0.854,respectively. Conclusion The serum level of LncRNA NORAD is high and miR-199a-3p is low in patients with esophageal squamous cell carcinoma.LncRNA NORAD and miR-199a-3p can both be used as an auxiliary diagnostic index for esophageal squamous cell carcinoma.

Key words: esophageal squamous cell carcinoma, serum, long non-coding RNA NORAD, micro RNA-199a-3p

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