关键词: miR-205, 10号染色体磷酸酶和张力蛋白同源缺失基因, 食管癌, 根治术, 预后

Abstract: Objective   To explore the expression of miR-205 and chromosome 10 phosphatase and tensin homologous deletion gene(PTEN) in esophageal carcinoma and their relationship with the prognosis after radical surgery.  Methods   78 patients with esophageal cancer undergoing radical resection admitted to the hospital from September 2018 to September 2019 were selected.Their paracancerous tissue and cancerous tissue samples were collected.Fluorescent quantitative PCR  was used to detect the expression of miR-205 and PTEN in adjacent and cancerous tissues.The relationship between the expression level of miR-205,PTEN and the pathological characteristics of esophageal cancer tissues was analyzed.All patients were followed up until September 30,2022.Kaplan Meier was used to analyze the relationship between the expression of miR-205,PTEN and the prognosis after radical resection of esophageal cancer.  Results   The expression level of miR-205 in cancer tissues was higher than that in adjacent tissues(P<0.05),and the expression level of PTEN was lower than that in adjacent tissues(P<0.05).The expression level of miR-205 was negatively correlated with that of PTEN(r=0.397,P<0.001).The expression level of miR-205 in cancer tissue of patients with lymph node metastasis,low differentiation,and stage Ⅲ to Ⅳ esophageal cancer was higher than that of patients without lymph node metastasis,high school differentiation,and stage Ⅰ to Ⅱ(P<0.05),and the expression level of PTEN was lower than that of patients without lymph node metastasis,high school differentiation,and stage Ⅰ to Ⅱ(P<0.05).All patients were followed for 3 years,a total of 32 patients died and 44 patients survived.The expression level of miR-205 in cancer tissues of the deceased patients was higher than that of the surviving patients(P<0.05),and the expression level of PTEN was lower than that of the surviving patients(P<0.05).Kaplan-Meier analysis showed that the difference of survival curve between patients with high and low expression of miR-205 and PTEN in cancer tissues was statistically significant,and patients with low expression of miR-205 and high expression of PTEN in cancer tissues had higher survival rates(P<0.05).  Conclusion   The expression levels of miR-205 and PTEN in cancer tissues of patients with esophageal cancer are closely related to the prognosis after radical surgery.The expression levels of miR-205 and PTEN in cancer tissues of patients with esophageal cancer were detected,which can be used as an important reference index to predict the prognosis after radical resection.

Key words: miRNA-205, phosphatase and tensin homolog deleted on chromosome ten, esophageal cancer, radical operation, prognosis