临床外科杂志 ›› 2022, Vol. 30 ›› Issue (2): 138-141.doi: 10.3969/j.issn.1005-6483.2022.02.012

• 论著 • 上一篇    下一篇

POU3F2在胶质瘤中的表达及其临床意义

  

  1. 430030 武汉,华中科技大学同济医学院附属同济医院神经外科
  • 收稿日期:2021-12-23 接受日期:2021-12-23 出版日期:2022-02-20 发布日期:2022-02-20
  • 通讯作者: 徐钰,Email:deliaxu@gmail.com

Clinical significance of POU3F2 expression in glioma

  1. Department of Neurosurgery,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030, China
  • Received:2021-12-23 Accepted:2021-12-23 Online:2022-02-20 Published:2022-02-20

摘要: 目的 研究POU3F2在胶质瘤中的表达及其临床意义。 方法 基于CGGA,GEPIA2,STRING,Metascape等数据库,使用R语言编程分析POU3F2基因在胶质瘤WHO高低级别组中表达的差异,采用免疫组化检测40例胶质瘤临床样本中POU3F2蛋白的表达水平进行验证;继而对POU3F2在不同胶质瘤分子亚型及分子标志物中表达差异及与预后的关系进行分析。 结果 POU3F2在高级别胶质瘤组中的表达量升高(P=0.0037)。免疫组化提示POU3F2在高级别胶质瘤组中表达量亦较高(P<0.001)。POU3F2在经典型胶质瘤中的表达量最高且与其他分子亚型差异显著(P<0.001),POU3F2表达水平预测经典型胶质瘤的AUC值为0.784。1p19q共缺失组胶质瘤中POU3F2表达量下调(P=0.0013)。 POU3F2高表达组预后较差(P=0.013)且在TCGA数据库中得到验证(P=0.0055)。〖HTH〗结论 POU3F2表达量的增加与恶性胶质瘤表型存在联系,POU3F2有望成为胶质瘤相关的分子标志物。 

关键词: POU3F2, 胶质瘤, 转录组, 分子标志物, 生物信息学

Abstract: Objective To explore the expression and significance of POU3F2 in glioma based on the Chinese Glioma Genome Atlas(CGGA). Methods Based on CGGA,GEPIA2,String,Metascape and other databases,we analyze the differences in the expression of POU3F2 gene in different WHO grade groups of glioma with R program language.Meanwhile,IHC staining was conducted to detect the protein expression level of POU3F2 in collected glioma tumor tissues.Then the relationships between POU3F2 gene expression and molecular subtypes of glioma,molecular biomarkers and clinical prognosis were analyzed. Results The expression of POU3F2 was increased in the high grade glioma group(P=0.0037).Immunohistochemistry indicated that POU3F2 expression was also up-regulated in high grade glioma group (P<0.001).The expression levels of POU3F2 was the highest in the classical glioma,and the difference was significant between other molecular subtypes (all P<0.001),and the AUC value of POU3F2 predicted the classical glioma was 0.784.The expression levels of POU3F2 gene were significantly lower in glioma with chromosome 1p19q co-deletion.High levels of POU3F2 expression tended to have a poorer prognosis(P=0.013)and was verified in the TCGA database(P=0.0055).Conclusion The increased expression level of POU3F2 is associated with malignant glioma phenotype,and might be a molecular biomarker of glioma.

Key words: POU3F2, glioma, transcriptome, molecular biomarker, bioinformatics

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