关键词: miR-711, 结肠癌干细胞, 上皮间质转化

Abstract: Objective To investigate the effect of miR-711 on invasion,invasion,proliferation and epithelial-mesenchymal transition of colon cancer stem cells.
Methods Colon cancer stem cells were isolated from human colon cancer cell lines HT-29 by flow cytometry.miR-711-mimics and miR-NC were transfected into colon cancer stem cells.qRT-PCR was used to detect the expression of miR-711 mRNA.MTT was used to detect cell proliferation.Transwell test was used to detect cell migration and invasion.Western blot was used to detect the protein expression of E-cadherin,N-cadherin,Vimentin.
Results The percentage of CD44+/CD133+in colon cancer cell line HT-29 was the highest(29.90%).The expression of miR-711 in over-expression group was higher than that in empty group and blank group(P<0.05).The proliferation,invasion ans migration ability of over-expression group was lower than those of empty group and blank group(P<0.05).The expression level of E-cadherin protein in over-expression group was higher than that of empty group and blank group(P<0.05).The expression level of N-cadherin,Vimentin protein in over-expression group were lower than those of empty group and blank group(P all<0.05).
Conclusion Up-regulation of miR-711 can inhibit the invasion,invasion,proliferation and epithelial mesenchymal-transition of colon cancer stem cells.

Key words: miR-711, colon cancer stem cells, epithelial mesenchymal transition