去乙酰化酶4在结直肠癌组织中的表达及其临床意义

doi:10.3969/j.issn.1005-6483.2020.02.014

摘要/Abstract

摘要：

目的分析去乙酰化酶4(Sirtuin 4,SIRT4)在结直肠癌组织中的表达及其临床意义。

方法分别采用RT-qPCR和Western blot检测SIRT4 mRNA和蛋白在正常结直肠细胞和结直肠癌细胞的表达水平；采用RTqPCR检测SIRT4 mRNA在结直肠癌组织和配对癌旁组织中的表达水平；进一步应用免疫组化技术检测SIRT4蛋白在85例结直肠癌组织中的表达情况，并分析其表达强度与临床病理特征及预后的相关性。

结果与正常结直肠细胞比较，SIRT4 mRNA和蛋白在结直肠癌细胞中的表达水平均显著降低，差异有统计学意义（P＜0.05）；在结直肠癌细胞中，SIRT4 mRNA蛋白的表达水平低于配对的癌旁组织，差异有统计学意义（P＜0.05）。免疫组化分析显示，SIRT4蛋白的低表达与结直肠癌的组织分化程度、肿瘤浸润深度、淋巴结转移、脉管浸润、神经侵犯及TNM分期显著相关（P＜0.05），与病人性别、年龄、肿瘤位置、肿瘤大小及术前CEA水平无关（P>0.05）。进一步生存分析显示，结直肠癌组织中SIRT4低表达与病人的不良预后显著相关（P＜0.05）。

结论 SIRT4在结直肠癌组织中表达降低，其低表达与病人的不良临床病理特征及不良预后密切相关，可能成为结直肠癌病人预后评估的生物标志物。

关键词: 结直肠癌, 去乙酰化酶4 , 病理特征, 预后

Abstract:

Objective To investigate the expressionof Sirtuin 4 (SIRT4) in colorectal cancer (CRC)cells and tissues and its clinical significance.

Methods RT-qPCR and Western blot were used to detect the expression of SIRT4 mRNA and protein in normal colorectal epithelial cell, CRC cells, CRC tissues and paired adjacent tissues, respectively. Then, the expression of SIRT4 protein in CRC tissues of 85 cases was investigated by immunohistochemical method, andthe correlation of SIRT4 expression with clinicopathological factors and patients' prognosis were further analyzed.

Results Compared to normal colorectal epithelial cell, the mRNA and protein expression of SIRT4 in CRC cells was significantly decreased(P＜0.05, respectively).Consistently, the mRNA and protein expression of SIRT4 in CRC tissues was significant lower than that of paired adjacent normal tissues(P＜0.05, respectively).Immunohistochemical analysis showed that the low expression of SIRT4 in CRC tissues were significant association with tumor grade, depth of invasion, lymph node metastasis,lympho-vascular invasion, nervous invasion and TNM stage (P＜0.05, respectively), but not with gender, age, tumor site,tumor size andpreoperative CEA level (P>0.05, respectively). Further Kaplan-Meier univariate analysis demonstrated that the low expression of SIRT4 protein was significant related to poor prognosis of CRC patients (P＜0.05).

Conclusion The low expression of SIRT4 in CRC is closely related to the unfavorable clinicopathological factors and poor patients' prognosis, indicating that SIRT4 may become a new biomarker to evaluate the prognosis of CRC.

Key words: colorectal cancer, SIRT4, clinicopathological factors, prognosis

王梦园, 左维维, 夏雨佳, 胡明. 去乙酰化酶4在结直肠癌组织中的表达及其临床意义[J]. 临床外科杂志, 2020, 28(2): 144-147.

WANG Mengyuan, ZUO Weiwei, XIA Yujia, et al.. The expression of sirtuin 4 in colorectal cancer and its clinical significance[J]. JOURNAL OF CLINICAL SURGERY, 2020, 28(2): 144-147.

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