Objective To report a case of giant retroperitoneal Castleman disease with tongue ulcers and cutaneous lesions and to improve the understanding of CD. Methods We retrospectively analyzed the clinical data of a case of CD,and discussed the clinical characteristics,diagnosis and treatment of this disease combined with latest literature. Results A 52-year-old woman who found a huge tumor behind the body and tail of pancreas by abdominal CT scan.According to her medical history and physical examination,she was accompanied with symptoms of intractable tongue ulcers and bipedal cutaneous lesions which were healed gradually by an operation of the complete tumor resection.The pathological examination showed that retroperitoneal CD and hyaline vascular subtype. Conclusion CD is a rare multi-clone lymphoproliferative disease without clear causes,which lacks specific clinical manifestations and imaging features,so CD is difficult to diagnosis.For abdominal masses with tongue ulcers and cutaneous lesions,it should be differentiated from CD.

Objective To explore the independent risk factors of early enteral nutrition aspiration in patients with severe acute pancreatitis (SAP) based on deep learning,and to establish a prediction model to predict the risk of early enteral nutrition aspiration. Methods The clinical data of 296 patients with severe acute pancreatitis in Tongji Hospital Affiliated to Tongji Medical College of Huazhong University of science and technology from January 2012 to December 2019 were retrospectively analyzed,including 268 patients without aspiration and 28 patients with aspiration.The clinical data of gender,age,body mass index,APACHE II score,consciousness,nutritional risk,nasogastric tube length,neutrophil-lymphocyte ratio (NLR) and platelet-lymphocyte ratio (PLR) were compared between the two groups Support vector machine (SVM) and generalized linear regression (GLR) algorithm were used to establish five prediction models to obtain the importance of prediction variables.At the same time,the subject work curve and decision curve were drawn to test the predictive value of the model. Results APACHE-II score,consciousness,nutritional risk,length of nasogastric feeding tube and PLR were the related variables to predict early enteral nutrition aspiration.The areas under the curve of random forest,neural network,decision tree,support vector machine and generalized linear regression algorithm were 0.976,0.973, 0.961 ,0.932 and 0.921,respectively.Through comparison,the performance of random forest algorithm was the best. Conclusion The prediction model based on machine learning algorithm can accurately predict the possibility of early enteral nutrition aspiration in patients with severe acute pancreatitis,which is conducive to postoperative evaluation and clinical nursing decision-making.

Objective To investigate the effect of stepped decompression under intracranial pressure monitoring on the inflammatory response,metabolic status and prognosis of patients with emergency craniocerebral trauma. Methods A total of 100 patients with trauma surgery  were randomly divided into two groups with 50 cases.The control group received conventional large bone flap decompression,and the observation group received stepped decompression.Inflammatory factors level were compared between 2 groups,and cerebral hemodynamics,protein metabolism and brain metabolism were monitored and compared between 2 groups. Results One week after operation,the inflammatory factors Monocyte chemoatrractant protein(MCP-1),hypersensitive C-reactive protein(hs-CRP),tumor necrosis factor(TNF-α)and interleukin 2(IL-2)in observation group were significantly lower，interleukin 2(IL-4)was higher than that in the control group.(P<0.05).The mean passage time(MTT) and peak time(TTP) of cerebral blood flow in the observation group were lower week and 1 month after surgery(P＜0.05),and the cerebral blood flow(CBF)andcerebral blood volume(CBV) were significantly higher(P＜0.05).One month after surgery,the expression levels of transferrin(TRE),retinol binding protein(RBP),and prealbumin(PA) in 2 groups were lower than before surgery,and the control group was significantly lower,cerebral metabolism [mixed venous oxygen saturation(SVO-2),cerebral oxygen uptake rate(CEO-2)] in 2 groups were all higher,and observation group was significantly higher(P<0.05).Conclusion Stepwise decompression under intracranial pressure monitoring can cause less injury to the body of patients with craniocerebral trauma,effectively reduce inflammatory response,and improve the metabolic state and prognosis.

Objective To investigate and compare the clinical effects of two different thoracoscopic surgery under DRGs in patients with spontaneous pneumothorax. Methods From April 1,2014 to May 30,2020,120 patients with primary pneumothorax who underwent thoracoscopic surgery in the Department of thoracic surgery,Hong Kong University-Shenzhen Hospital.All patients underwent thoracoscopic surgery.They were divided into low-cost group and conventional group according to whether low-cost thoracoscopic surgery was used or not during the operation.The time required for surgery,postoperative thoracic drainage tube indwelling time,postoperative hospitalization days,total cost of surgery and related consumables,total hospitalization costs,postoperative complications and recurrence rate of pneumothorax were calculated respectively. Results All patients successfully completed the operation without death or serious complications.The comparison of total hospitalization cost between experimental group and control group showed significant difference（P<0.05）,The average and IQR values were 12 998 yuan(10 645,14 427 yuan) versus 32 121 yuan(25 383,33 152 yuan).There were significant differences in the cost of operation and related consumables(P<0.05).The average and IQR value was 8 104 yuan(6 430,7 247 yuan) to 26 158 yuan(20 492,27 256 yuan).There was no significant difference in the number of postoperative complications and pneumothorax recurrence between the two groups（P＞0.05）. 〗Conclusion The clinical application of low-cost VATS in the treatment of spontaneous pneumothorax is safe and feasible.Compared with the conventional VATS group,it can significantly reduce the cost of patients.

Objective To compare with laparoscopic-assisted total gastrectomy,to evaluate the safety and efficacy of total endoscopic esophagojejunostomy in radical total gastrectomy. Methods The clinical data of 70 patients who underwent 3D laparoscopic radical total gastrectomy from January to July 2019 in our hospital were retrospectively analyzed.The observation group(n=30) underwent total laparoscopic  π-shaped esophagojejunostomy,the control group(n=40) underwent laparoscopic-assisted end-to-side esophagojejunostomy.The operation time,blood loss,anastomosis time,incision length,postoperative pain,postoperative exhaust time,feeding time,hospitalization time and postoperative complications were compared between the two groups. Results R0 resection was performed in both groups.There were no significant differences in age,sex,BMI,pathological features(depth of tumor invasion,regional lymph node metastasis,number of lymph node dissection,degree of tumor differentiation,nerve invasion and vascular invasion) between the two groups(P＞0.05) .In the observation group, there was no perioperative death, anastomotic leakage and other complications, including 1 case of pleural effusion and lung infection, and 1 case of pneumothorax. In the control group, anastomotic leakage, abdominal cavity infection, incision infection and lung infection occurred in 1 case each.The postoperative VAS pain score, surgical incision length and intraoperative anastomosis time of the observation group were better than those of the control group, and the differences between the two groups were statistically significant(P＜0.05).There were no significant differences in operation time,blood loss,incision length,postoperative ventilation time,postoperative feeding time and postoperative hospital stay between the two groups(P＞0.05). Conlusion 3D laparoscopic esophagojejunostomy is safe and effective,with less trauma,less postoperative pain,and does not increase the incidence of postoperative complications and other advantages.

Objective To investigate the feasibility of pancreaticoduodenectomy in elderly patients over 80 years old. Methods A retrospective analysis of 58 patients with pancreaticoduodenectomy performed in Songjiang Hospital Affiliated to Shanghai Jiaotong University School of Medicine from March 2017 to October 2020.Taking 80 years old as the classification standard,28 people aged 80 and above belong to the senior group,and 30 people aged 80 and below belong to the non-elderly group.The preoperative complications,surgery-related conditions and postoperative complications were compared between the two groups. Results The preoperative comorbidities of the two groups were compared.There were 13 cases of hypertension,7 cases of heart disease,9 cases of diabetes mellitus and 16 cases of hypoalbuminemia in the elderly group,and 7 cases,4 cases,3 cases and 7 cases in the non-senior age group,respectively.The difference was statistically significant(P<0.05).Comparison of operation-related indicators between the two groups,the operation time in the elderly group was(172.4±33.2) minutes,and the intraoperative blood loss was(160.0±84.4) ml,while the non-elderly group was(160.6±35.8) minutes and(156.6±84.8) ml,respectively.There was no significant difference between groups(P>0.05).The postoperative hospital stay days in the elderly group were(22.6±9.0) days and in the non-elderly group(18.9±2.8) days,there was a statistically significant difference between the two groups(P<0.05).The postoperative complications,including pancreatic fistula,bile leakage,intra-abdominal infection,delayed gastric emptying and intra-abdominal hemorrhage,showed no significant difference between the two groups(P>0.05).In the comparison of prognostic indicators,there was 1 case of severe pancreatic fistula-induced hemorrhage death in the elderly group,and no death in the non-elderly group,and the difference was not statistically significant(P>0.05).Conclusion Pancreaticoduodenectomy in elderly patients requires adequate preoperative preparation,meticulous intraoperative operation,and effective postoperative management to prevent complications.Even for elderly patients over 80 years old,the operation is safe and feasible.

Objective Investigate the safety,feasibility and efficacy of emergency bedside endoscopic nasobiliary drainage(ENBD) in supine position without fluoroscopy in ICU for patients with severe acute cholangitis and septic shock. Methods In total,31 patients with severe acute cholangitis underwent emergency bedside ENBD in supine position without fluoroscopy in ICU from January 2012 to June 2018.The clinical datas were reviewed and analyzed. Results Endoscopic nasobiliary drainage was successful in all 31 patients,the operation success rate was 100%,the operation time was 27.23 minutes.High fever,inflamation and jaundice were quickly degraded,and heart rate and blood pressure regained steady soon after ENBD.There were 27,2 and 1 cases of patients with high fever(T≥39℃) on three different time points(before,1day and 2 days after ENBD),respectively.The proportions were respectively 87.1%,6.5% and 3.2%（P<0.05）.There were respectively 31,8 and 3 cases of patients with the fastest heart rate more than 100 beats per min on three different time points.The proportions were respectively 100%,25.8% and 9.7%（P<0.05）.There were respectively 31,10 and 2 cases of patients with hypotension requiring vasopressors of any dose on three different time points.The proportions were respectively 100%,32.3% and 6.5%（P<0.05）.There were respectively 28,12 and 4 cases of patients with WBC count more than 20×10^9/L on three different time points.The proportions were respectively 90.3%,35.5% and 12.9%（P<0.05）.There were respectively 29,12 and 7 cases of patients with total bilirubin(TB) level more than 100 μmol/L on three different time points(before,3 days and 5days after ENBD).The proportions were respectively 93.5%,38.7% and 22.6%.The proportions on 3days and 5days after ENBD compared with that before ENBD were declined significantly（P<0.05）.Twenty-nine patients were cured.Two patients were dead,Mortality rate was 6.5%.There was no ENBD-related complications. Conclusions Emergency bedside ENBD in supine position without fluoroscopy in ICU was safe and feasible.Without heavy X-ray machine,transporting patients,changing their position and through normal tract it possessed the least interference and trauma to patients and was a prompt and effective method of biliary drainage.

ObjectiveTo investigate the effect of double acting total hip arthroplasty in the treatment of traumatic femoral neck fracture.Methods The clinical data of 65 patients with total hip arthroplasty in the Department of orthopedics of Linfen Central Hospital from January 2018 to December 2020 were collected.27 patients with double acting total hip arthroplasty were used as the observation group and 38 patients with traditional total hip arthroplasty were used as the control group,the observation group was treated with double acting total hip arthroplasty,and the control group was treated with traditional total hip arthroplasty.The operation indexes,hip function and activities of daily living,quality of life,range of motion of hip joint and incidence of complications were compared and analyzed between the two groups.Result The improvement of surgical indexes in the observation group was better than that in the control group(P<0.05).The hip function,ADL score and quality of life score of the two groups were increased at 3 and 6 months after treatment.The score in the observation group was higher than that in the control group,and the score at 6 months after treatment was higher than that at 3 months after treatment(P<0.05).There was no significant difference in combined anteversion between the two groups at 6 months after treatment(P>0.05),the activities of internal and external rotation,internal and external exhibition and flexion and extension in the observation group were higher than those in the control group,and the incidence of complications in the observation group was lower than that in the control group(both P<0.05). Conclusion The treatment of traumatic femoral neck fracture with double acting total hip arthroplasty can effectively improve the surgical indexes and hip function,improve the ability of daily life and quality of life,and reduce the incidence of complications.

Objective  To explore the efficacy of three surgical postures in the treatment of traumatic femoral intertrochanteric fractures. Methods 98 patients with traumatic femoral intertrochanteric fracture treated in our hospital from February 2017 to June 2020 were selected.According to different surgical positions,they were divided into lithotomy position group(33 cases),supine position group(33 cases) and lateral position group(32 cases).The three groups were treated with Asian proximal femoral anti rotation intramedullary nail.The operation related indexes,excellent and good rate of hip function,pain,anxiety and complications were compared. Results There was no significant difference in intraoperative bleeding and fracture healing time among the three groups(P>0.05).The placement time,operation time and fluoroscopy time of lithotomy position group were shorter than those of Supine position group and lateral lying position group(P<0.05).During the follow-up at 6 months after operation,the excellent and good rate in the lateral position group(90.63%) was higher than that in the lithotomy group(84.85%) and supine group(72.73%),but there was no significant difference among the three groups(P>0.05).Compared with the same group before operation, the VAS scores of the three groups were significantly improved at 3 days and 6 months after operation (P<0.05). There was no significant difference in VAS scores before and after operation among the three groups (P>0.05).However,  the score of Amsterdam Preoperative Anxiety and Information Scale (APAIS score) of the three groups had statistical significance in 30 minutes (P<0.05), and the supine position group and lateral position group were higher than the lithotomy position group (P<0.05).The short-term complications in the three groups were 6.06%,9.09% and 6.06%,respectively,and the long-term complications were 3.03%,6.06% and 3.13%,respectively.There was no significant difference in the short-term and long-term complications among the three groups(P>0.05). Conclusion Lithotomy position,Supine position and lateral decubitus position are effective in the treatment of traumatic femoral intertrochanteric fracture,with better recovery of hip function,less pain and fewer complications.However,the lithotomy position is helpful to reduce the injury to doctors and patients and patients' anxiety caused by repeated fluoroscopy,so as to shorten the operation time.

Objective To compare the short- and long-term effects of unicondylar replacement with medial fixed platform and movable platform on spontaneous osteonecrosis of the knee(SONK). Methods A retrospective analysis of 72 SONK patients who underwent unicondyle replacement in our hospital from February 2016 to March 2020 was divided into medial fixed platform group(35 cases) and movable platform group according to the difference of the unicondyle replacement prosthesis used by the patients(37 cases).The operation time,intraoperative blood loss,knee range of motion(ROM),Knee Society(KSS) score,American Hospital for Special Surgery(HSS) score and lower extremity alignment were compared between the two groups.After a 2-year follow-up,the prosthesis survival of the two groups was analyzed.Results There was no significant difference in the operation time and intraoperative blood loss between the two groups(P>0.05).At 6 months after operation,the ROM,KSS score,HSS score,hip,knee and ankle angle of the two groups were all increased(P < 0.05),while the tibiofemoral angle was decreased(P<0.05).There was no significant difference in ROM,KSS score,HSS score,hip-knee-ankle angle,tibiofemoral angle and tibial retroversion angle between the two groups at 6 months after operation(P>0.05).There was no significant difference in the survival curve of prosthesis between the two groups(P>0.05). ConclusionThe treatment of SONK with medial fixed platform and movable platform unicondyle replacement is quite effective.

Objective To explore the relationship between circulating miR-485-5p levels and pathological parameters of prostate cancer patients and its prognostic value. Methods A prospective study was conducted on 103 patients with prostate cancer admitted to the hospital from October 2016 to November 2018.All patients underwent radical surgery for prostate cancer and were followed up for 3 years.The relative expression of miR-485-5p was detected in all patients before operation.The relative expression of miR-485-5p in serum of prostate cancer patients with different pathological parameters was analyzed.Factors affecting the recurrence and metastasis of prostate cancer patients were analyzed.The value of serum miR-485-5p relative expression in predicting postoperative recurrence and metastasis of prostate cancer patients was analyzed.The relationship between the relative expression of miR-485-5p and the prognosis of prostate cancer patients was analyzed. Results The relative expression of miR-485-5p in patients with Gleason score>7,microvascular invasion,lymph node metastasis,and stage Ⅲ was lower than Gleason score ≤7 points,no microvascular invasion,no lymph node metastasis,and stage Ⅰ-Ⅱ(P<0.05).As of the end of follow-up,10 patients were lost to follow-up,and 35 of the remaining 93 prostate cancer patients had recurrence and metastasis.Cox regression analysis showed Gleason score,stage,relative expression of miR-485-5p was independent factor affecting postoperative recurrence and metastasis of prostate cancer patients(P<0.05).ROC curve results showed that the sensitivity of serum miR-485-5p level to predict postoperative recurrence and metastasis of prostate cancer patients was 74.29%,and the specificity was 75.86%,AUC was 0.795.The best cut-off point(1.02) for predicting postoperative recurrence and metastasis of prostate cancer patients with the relative expression of preoperative serum miR-485-5p is the dividing line.41 patients with low miR-485-5p expression survived 21 patients,and 52 patients with high miR-485-5p expression survived 37 patients;patients with high miR-485-5p expression had better disease-free survival curves than miR-485 For patients with low expression of -5p(P<0.05). Conclusion The expression of miR-485-5p is related to the pathological parameters and prognosis of prostate cancer patients.Patients with low miR-485-5p expression have a high risk of poor prognosis.

Objective To study the correlation between phosphodiesterase 5(PDE5) gene polymorphism and penile erectile dysfunction(ED) and its role mechanism. Methods 107 patients with ED and 102 healthy males with physical examination in the hospital between January 2015 and December 2020 were selected as observation group and control group respectively.The basic data were collected in the two groups,and the PDE5 gene polymorphism and levels of serum nitric oxide(NO) and cyclic guanosine monophosphate(cGMP) were detected and compared,and the relationship between each indicator and International Index of Erectile Function 5(IIEF-5) score was analyzed. Results The proportions of T allele and TT genotype at rs3806808 locus in ED group were lower than those in control group(P<0.05).There was a significant difference in the severity of ED among patients with different genotypes at rs3806808 locus of PDE5(P<0.05).The proportion of severe patients in TG and GG groups was lower than that in TT group,while the proportion of mild-to-moderate patients was higher than that in TT group(P<0.05).There was no significant difference in serum NO level in patients with different genotypes at rs3806808 locus of PDE5(P>0.05),and the level of serum cGMP in TG and GG groups was lower than that in TT group while the IIEF-5 score was higher than that in TT group,and the level of serum cGMP in GG group was lower than that in TG group while the IIEF-5 score was higher than that in TG group(P<0.05).Multivariate linear regression analysis showed that serum NO and genotypes at rs3806808 locus of PDE5 were important factors affecting IIEF score in patients with ED(P<0.05).Conclusion Gene polymorphism of PDE5 rs3806808 locus is closely related to the pathogenesis and progression of ED,and its role mechanism may mainly affect the expression of cGMP,but has no obvious relationship with the expression of NO.

Objective To compare the two micro-varicocelectomy method，investigate the best methods of operation. Method A total of 191 patients with varicocele admitted to our hospital from May 2016 to May 2019 were randomly divided into two groups using the random number table method. Group A(n=72) was selected through the superficial inguinal ring approach, and group B (n=119) was selected through the inguinal approach.Compare their operative time,number of vein,number of lymphatics, side effects,results after operation,spouse partner rate of pregnancy. Result Length of incision:group A：(1.5±0.30)cm，group B：(2.0±0.35)cm(P＜0.05).Operation time:group A：(40.5±6.8)min,group B：(30.2±4.8)min(P＜0.05).Vein ligation during operation:group A:mean(10±6.3)branches;group B:mean(7.2± 3.3)branches(P＜0.05).Lymphatics observed:group A：mean(4±2.3)branches；group B:mean(2.5±1.3) branches(P＜0.05).There was no testicular atrophy in both groups after operation, and 2 cases in group A had mild testicular hydrocele. The semen quality of the two groups was significantly improved after operation compared with that before operation, and the difference was statistically significant(P＜0.05).Sperm period comparison between the two groups, there was no statistically significant difference (P>0.05). Group A recent recurrence in 1 case, 2 cases, group B is similar between the two groups have no statistical significance (P>0.05). There were 33 pregnant spouses in group A and 63 pregnant spouses in group B. There was no significant difference between the two groups(P＞0.05). Conclusion Two methods of microsurgery varicocelectomy are safe and effective for the treatment of varicocele.Superficial inguinal ring approach might be used to fat or second operation patients.The number of veins ligated through inguinal approach is less and the operation time is short, which is suitable for ordinary patients.

Objective To introduce the advantages of a kind of dual-channel ureteroscope in the treatment of holmium laser lithotripsy for upper ureteral calculi in children. Methods 80 cases with  unilateral ureteral calculi admitted to our hospital from April 2020 to December 2020 were retrospectively analyzed.40 patients in the experimental group were treated with pediatric dual-channel ureteroscopy for holmium laser lithotripsy,and other 40 patients in the control group were treated with conventional single-channel ureteroscopy for holmium laser lithotripsy.Compared  the success rate of ureteroscopy insertion,operation time,postoperative fever and other complications,escape rate and clearance rate of alculi. Result The ureteroscope was successfully inserted in all the patients of the experimental group(100% vs 77.5%),the difference was statistically significant(P＜0.05).Experimental calculi escape rate (2.5%) is far lower than the control group (32.5%), the difference was statistically significant(P＜0.05).Experimental operation time for (31.8±2.23) minutes, and the control group (48.6±3.71) minutes, the more similar between the two groups was statistically significant (P＜0.05). Experimental group of stone clearance rate (100%) than the control group (80%), difference was statistically significant (P＜0.05). Conclusion Dual-channel reteroscopy is simple in operation,with clear field of vision in the process of lithotripsy,short operation time,low stone escape rate,high lithotripsy efficiency,less postoperative complications,and low price.

Objective To explore the changes and significance of serum Notch1 and reduced upon activation mormal T expression and secreted (RANTES) in the formation of abdominal aortic aneurysm (AAA). Methods From January 2019 to January 2021,41 patients with AAA were selected as the AAA group.41 healthy volunteers during the same period were selected as the healthy group.The levels of Notch1 mRNA and RANTES in serum were detected by RT-qPCR and ELISA.The expression levels of Notch1 and RANTES proteins in AAA tissues were detected by Western blot.Pearson was used to detect the correlation of continuous variables. Results The levels of Notch1 mRNA and RANTES in serum of AAA patients were (3.85±0.62) and (58.46±5.59)μg/L,which were significantly higher than those of healthy individuals[ (1.04±0.23),(21.07±3.25)μg/L]（P<0.05）.The expression levels of Notch1 and RANTES protein in AAA tissue were (3.24±0.65) and (3.41±0.72) ,which were significantly higher than those in normal abdominal aorta tissue [(1.08±0.21),(1.12±0.23)]（P<0.05）.The level of Notch1 protein in AAA tissue was positively correlated with the level of Notch1 mRNA in serum of AAA patients （r=0.485,P=0.025）,and the level of RANTES protein in AAA tissue was positively correlated with the concentration of RANTES in serum（r=0.506,P=0.016）.In AAA tissue and serum of AAA patients,Notch1 and RANTES showed a positive correlation （r-serum=0.515,P=0.014；r-tissues=0.537,P=0.008）. Conclusion Notch1 and RANTES are over-expressed in serum and tissues of AAA patients,and the two are positively correlated.

Objective To investigate the analysis of clinical efficacy、hemodynamic indexes and prognosis of laparoscopic splenectomy and Endoscopicvariceal ligation and sclerotherapy in the treatment of portal hypertension patients. Methods From August 2015 to January 2018, 91 patients with PHT were admitted,according to the treatment were divided into control group(n=45) and group(n=46),the control group using cardiac blood vessels around from surgery combined therapy with laparoscopic splenectomy,the team used laparoscopic splenectomy in combination with endoscopic sequential ligation and hardener treatment,compared two groups of clinical curative effect. Results The intraoperative bleeding(241.1±82.0)ml,postoperative abdominal drainage(473.8±122.3)ml,postoperative anal venting time(2.1±0.5)d,awake rate(15.2%) and postoperative hospital stay(11.3±2.2)d in the study group were better than those in the control group[(363.4±118.2)ml,(677.5±154.0)ml,(3.5±0.9)d,35.6% and (16.0±3.4)d](P<0.05).The postoperative serum inflammatory indexes[TNF-α:(38.1±9.3)ng/L,IL-6:(27.6±7.2)ng/L and CRP:(41.6± 11.2)mg/L] and hemodynamic parameters[PVP:(21.5±1.5)mmHg,PVV:(18.7±2.7)cm/s,PVD:(11.4±0.5)mm and PVF:(535.7±90.6)ml/min] compared with the control group[TNF-α:(73.0±11.1)ng/L,IL-6:(40.3±4.4)ng/L and CRP:(68.4±12.0)mg/L;PVP:(25.4±1.8)mmHg,PVV:(19.4±3.0)cm/s,PVD:(13.2±0.6)mm and PVF:(739.4±93.8)ml/min](P<0.05).After six months,there was not statistically significant in sithe Child-Pugh score compared with the two groups(P>0.05). Conclusion The laparoscopic splenectomy and EVLS therapy in the treatment of portal hypertension patients has a better therapeutic effect, which can not only reduce the inflammatory response of patients, but also have little influence on hemodynamics and prognosis.

Objective To investigate the mechanisms of GATA3/GATA6 regulating autophagy and proliferation of colon cancer cell lines.〖WTHZ〗Methods Colon cancer cell line HCT116 were used as experimental materials,and transfected with the vectors expressing small interfering RNAs targeting GATA3 and GATA6 and the negative control vectors,respectively.Flow cytometry was used to detect cell cycle changes in the transfected cells,Annexin-V-FITC/PI double staining was used to detect apoptosis,and MDC staining was applied to measure cell autophagy. Results Flow cytometry showed that the apoptosis rate of colon cancer cells after GATA3/GATA6 interference[(43.15±13.58)%] was significantly higher than that of blank control group[(4.13±0.46)%,P<0.05].In the control group, the proliferation levels of colon cancer cells at 0h, 24h, 48h and 72h were 1.05±0.06、1.01±0.07、1.03±0.04、1.06±0.07,respectively.After GATA3/GATA6 interference,the data were 1.04±0.04、0.81±0.03、0.61±0.06、0.49±0.05,respectively. Compared with the control group, the survival rate of colon cancer cells decreased significantly, and showed a downward trend over time（P<0.05).The mRNA expression levels of mTOR, ULK1 and LC3-B in colon cancer cells after GATA3/GATA6 interference were 0.62±0.13、0.51±0.06、0.37±0.04,respectively,which were significantly decreased compared with the control group(0.92±0.16、0.86±0.13、0.82±0.14,respectively)(P<0.05).After interfering GATA3/GATA6,the protein expression levels of mTOR,ULK1 and LC3-B in colon cancer cells were significantly lower than those in control group(P<0.05). Conclusion Interfering GATA3/GATA6 may inhibit autophagy and cell proliferation in colon cancer cells,and increase apoptosis.