Abstract:   Objective  To observe the occurrence of anastomotic fistula after laparoscopic colorectal cancer resection with indocyanine green imageing，and to determine whether indocyanine green imaging is effective in preventing laparoscopic colorectal cancer anastomotic fistula. Methods  Randomly selected 145 patients with laparoscopic colorectal cancer resection，82 patients in control group，63 patients with indocyanine green imageing（ICG group）.The time of operation,the amount of bleeding during operation,the number of anastomotic leakage and the number of deaths caused by anastomotic leakage were observed. Results   In the control group，there were 7 patients with anastomotic fistula（2 with left hemitomy，1 with right hemicolectomy,4 with low rectal resection）and 1 case died from anastomotic leakage.In ICG group，only one patient undergoing lower rectal resection had anastomotic fistula，and none dead case.There was a significant difference between the two groups in the incidence of anastomotic leakage（f=2.337，P<0.05）. Conclusion Indocyanine green imaging can be used to reduce postoperative anastomotic leakage with laparoscopic colorectal cancer surgery.