Abstract: Objective To analyze the relationship between preoperative serum cystatin 6 (CST6),centromere protein A (CENPA),C-type lectin domain family 3 member B (CLEC3B) and clinical features and prognosis in bladder cancer.Methods From January 2020 to December 2021,160 patients with bladder cancer who were treated in our hospital were collected for research (cancer group),and 160 healthy people were served as control group.The levels of serum CST6,CENPA and CLEC3B were detected by enzymelinked immunosorbent assay.The receiver operating characteristic (ROC) curve was used to analyze the value of serum CST6,CENPA and CLEC3B in predicting the prognosis of bladder cancer.The relationship between serum CST6,CENPA,CLEC3B and prognosis was analyzed using the Kaplan-Meier-method.Results The levels of CST6 and CENPA in the cancer group were (7.65±1.59) ng/ml and (15.41±4.67) ng/ml respectively,which were higher than those in the control group [(3.24±0.97) ng/ml and (8.92±2.63) ng/ml].The CLEC3B in the cancer group was (67.29±13.25) ng/ml,which was lower than that in the control group [(95.35±15.96) ng/ml],and the difference between the two groups was statistically significant (P<0.05).The serum CST6,CENPA,and CLEC3B was statistically clearly different among different differentiation,T staging,and lymph node metastasis (P<0.05).The poor prognosis group had higher CST6 and CENPA,and lower CLEC3B than good prognosis group (P<0.05).The ROC curve showed that the area under the curve (AUC) of the combined prediction of patient prognosis by CST6,CENPA and CLEC3B was 0.926,which was significantly higher than that of the individual predictions of the three.Kaplan-Meier analysis showed that there were significant differences in cumulative survival rates among those with high and low expressions of serum CST6,CENPA,and CLEC3B (Log Rankχ2=11.102,15.817,36.612,all P<0.001).Conclusion CST6,CENPA and CLEC3B are all related to the clinical characteristics and prognosis of patients with bladder cancer.The combination of the three has a high value in predicting the prognosis of bladder cancer and is related to the overall survival rate after bladder cancer surgery.

Key words: bladder cancer, cystatin 6, centromere protein A, C-type lectin domain family 3 member B