Abstract: Objective To investigate the expression of silencing information regulatory factor 3 (SIRT3) in lung cancer tissues and its effects on lung cancer cell proliferation,invasion and migration.Methods The tumor tissues and adjacent normal lung tissues of 80 lung cancer patients admitted from October 2022 to October 2024 were randomly selected,then positive expression rate and protein expression of ,IL-6,STAT3 and SIRT3 were detected by immunohistochemistry staining and Western blot,respectively.Lung cancer cell lines A549 and SK-MES-1 were selected,SIRT3 over-expression,low-expression and empty vector lentiviruses in vitro were constructed,then were transfected to cells for 48 hours.MTT assay was used to detect cell proliferation,Transwell assay was used to detect cell invasion and cell scratch assay was used to detect cell migration.Results Compared with normal tissues adjacent to cancer,positive expression rate and protein expression of SIRT3,IL-6 and STAT3 in tumor tissues were significantly lower(P<0.05).Compared with empty vector group,over-expression group showed significant decreases in cell proliferation,invasion and migration,while low-expression group showed significant increases(P<0.05).Conclusion Low expression of SIRT3 may be involved in the occurrence and development of lung cancer,which may be related to inhibition of IL-6/STAT3 signaling pathway.

Key words: silent information regulation factor 3, lung cancer, proliferation, invasion, migration