临床外科杂志 ›› 2018, Vol. 26 ›› Issue (10): 747-750.doi: 10.3969/j.issn.10056483.2018.10.009

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miR31促进结肠癌转移侵袭的作用及机制探讨

  

  1. 430064 中国人民解放军武汉总医院普通外科
  • 收稿日期:2018-03-07 出版日期:2018-10-20 发布日期:2018-10-20
  • 通讯作者: 张杨,Email:zhangyang_0005@sina.com

The effect and mechanism of miR31 on the metastasis and invasion of colon cancer

  1. Department of General Surgery,Wuhan General Hospital of the Chinese people's Liberation Army,Wuhan 430064,China  
  • Received:2018-03-07 Online:2018-10-20 Published:2018-10-20

摘要: 目的  探讨微小RNA31(miR31)在结肠癌组织中的表达及与预后的相关性及对结肠癌细胞侵袭转移的作用及机制。方法 因原发性结肠癌行手术治疗的病人病理标本43例,采用实时定量PCR(quantitative realtime PCR,qRTPCR)检测结肠癌组织miR31的表达,分析miR31表达与病人预后与病理分级等之间的关系。采用脂质体介导转染方法,分别用miR31mimic与miR31阴性对照转染人结肠癌HT29细胞系。划痕实验检测miR31对细胞迁移能力的影响;Transwell实验检测miR31对细胞侵袭能力的影响。Westernblot检测miR31对Wnt/βcatenin信号通路及重要靶基因Claudin1的影响。结果  结肠癌TNM分期Ⅰ~Ⅱ期病人miR31的表达为1.78±0.25 ,Ⅲ~Ⅳ期病人为2.35±0.41;肿瘤穿透浆膜病人miR31的表达为2.34±0.47 ,未发生转移病为1.67±0.35;发生淋巴结转移病人miR31的表达为3.49±0.42,未发生淋巴结转移病人为1.64±0.71;发生脉管侵犯病人miR31的表达为3.31±0.58,未发生脉管侵犯病人为2.18±0.63,差异均有统计学意义(P<0.05)。人结肠癌HT29细胞中,miR组愈合面积比例为0.74±0.16,阴性对照(NC)组为 0.43±0.21;miR组穿膜细胞数量为182.7±29.3 ,NC组为139.2±25.6;miR组上皮标志物Ecadherin的表达为0.51±0.24,NC组为0.89±0.19;βcatenin的激活表达为1.01±0.23,NC组为0.65±0.24;Claudin1的表达0.46±0.17,NC组为0.21±0.12,差异均有统计学意义(P<0.05)。〖HTH〗结论〓〖HTSS〗miR31通过异常激活Wnt/βcatenin信号通路,导致Claudin1的异常表达,促进结肠癌的侵袭、迁移,影响结肠癌细胞的上皮间质转换(EMT)。在结肠癌中,miR31可能发挥促进肿瘤的增值、迁移和侵袭作用。
[关键词]  miR31; 结肠癌; 侵袭; Wnt/βcatenin信号通路; Claudin1

Abstract: 〓Objective  To investigate the expression of micro RNA31(miR31)in colon cancer tissue and its correlation with prognosis,and to study the effect and mechanism of the invasion and metastasis of colon cancer.Methods Pathological specimens of 43 patients undergoing surgical treatment for primary colon cancer.Real time quantitative PCR(quantitative realtime PCR)was used to detect the expression of miR31 in colon cancer tissue,and the relationship between miR31 expression and prognosis and pathological grading was analyzed.The transfection method of liposome mediated transfection was used to transfect human colon cancer HT29 cell line with miR31mimic and miR31 negative control respectively.The effect of miR31 on cell migration ability was detected by scratch test,and the effect of miR31 on cell invasiveness was detected by Transwell test.The effects of miR31 on the Wnt/ betacatenin signaling pathway and the important target gene Claudin1 were detected by Westernblot.〖WTHZ〗Results〖WTBZ〗〓The colon cancer TNM stage Ⅰ ~ Ⅱ expression of miR31 patients was 1.78±0.25,while in patients with stage Ⅲ~Ⅳ was 2.35±0.41(P<0.05).The expression of tumor penetrating serosa in patients with miR31 was 2.34±0.47,while that in patients without metastasis was 1.67±0.35(P<0.05).The expression of miR31 in patients with lymph node metastasis was 3.49±0.42,expression of miR31without lymph node metastasis was1.64±0.71(P<0.05).The expression of vascular invasion in patients with miR31 was 3.31±0.58,that without the occurrence of vascular invasion patients was 2.18±0.63(P<0.05).In human colon cancer HT29 cells,the percentage of healing area in miR group(0.74±0.16)was significantly higher than that in NC group(0.43±0.21),the difference was statistically significant(P<0.05).The number of membrane cells in group miR(182.7±29.3)was significantly more than that in group NC(139.2±25.6,P<0.05).The expression of epithelial marker Ecadherin in miR group(0.51±0.24)was significantly lower than that of NC group(0.89±0.19,P<0.05).In addition,activation of betacatenin expression was significantly higher in miR group(1.01±0.23)than that of NC group(0.65±0.24,P<0.05).The expression of Claudin1 was significantly higher in miR group(0.46±0.17)than that of group NC(0.21±0.12;t=2.69,P<0.05).Conclusion  miR31 abnormal activates the Wnt/catenin signaling pathway,resulting in overexpression of Claudin1,promoting colon cancer invasion and migration,and affecting the EMT.In colon cancer,miR31 may play a role in promoting cancer.

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