关键词: LncRNA NEAT1； miR-138-5p； 乳腺癌； 临床病理参数； 预后

Abstract: Objective To explore the correlation between the expression of long non coding RNA nuclear paraspeckle assembly transcript 1(LncRNA NEAT1),microRNA(miR)-138-5p and prognosis in breast cancer.Methods 160 breast cancer patients undergoing surgical treatment from january 2022 to january 2023.QRT-PCR was used to detect the expression levels of LncRNA NEAT1 and miR-138-5p in breast cancer tissues and adjacent tissues.Pearson method was used to analyze the correlation between LncRNA NEAT1 and miR-138-5p levels in breast cancer tissues.The predictive efficacy of LncRNA NEAT1 and miR-138-5p levels on recurrence and metastasis of breast cancer patients was analyzed by establishing receiver operator characteristic (ROC) curve.Results Compared with the adjacent tissues,the expression level of LncRNA NEAT1 in breast cancer tissues increased (P<0.05),and the expression level of miR-138-5p decreased (P<0.05).The levels of LncRNA NEAT1 and miR-138-5p in breast cancer tissues with different lymph node metastasis,TNM stage and differentiation degree were statistically significant (P<0.05).TargetScanHuman website predicted that there was a targeted binding site between LncRNA NEAT1 and miR-138-5p,and the expression level of LncRNA NEAT1 and miR-138-5p in breast cancer tissue was negatively correlated (r=-0.529,P<0.05).Compared with the non recurrence and metastasis group,the expression level of LncRNA NEAT1 increased and the expression level of miR-138-5p decreased in the recurrence and metastasis group (P<0.05).The area under the curve (AUC) of LncRNA NEAT1 combined miR-138-5p in predicting the recurrence and metastasis of breast cancer patients was 0.928,which was better than that of LncRNA NEAT1 and miR-138-5p separately (Z_{combination-LncRNA NEAT1}=3.489,P=0.001,Z_{combination-miR-138-5p}=4.235,P<0.001).Conclusion The expression level of LncRNA NEAT1 in breast cancer tissue increases and the expression level of miR-138-5p decreases,which are closely related to the clinical pathological parameters.The combination of the two can better predict the recurrence and metastasis of breast cancer patients.

Key words: LncRNA NEAT1; microRNA-138-5p; breast cancer; clinical pathological parameters; prognosis