LIM激酶1对肝癌细胞增殖、迁移及血管形成能力的影响

doi:10.3969/j.issn.1005-6483.2023.10.006

临床外科杂志 ›› 2023, Vol. 31 ›› Issue (10): 920-924.doi: 10.3969/j.issn.1005-6483.2023.10.006

LIM激酶1对肝癌细胞增殖、迁移及血管形成能力的影响

430071 武汉大学中南医院消化内科，湖北省肠病医学临床研究中心，肠病湖北省重点实验室(劳垚佳、程洁、林梦璐、奉佳辉、牟娟丽、黄凯、胡凡、林军) ；江汉大学附属中山医院消化内科(张海平)

收稿日期:2022-12-26 接受日期:2022-12-26 出版日期:2023-10-20 发布日期:2023-10-20
通讯作者: 通信作者：林军,Email：linjun64@126.com

Effect of LIM kinase 1 on the proliferation,migration and ability to promote angiogenesis of hepatocellular carcinoma cells

Department of Gastroenterology,Zhongnan Hospital of Wuhan University,Hubei Clinical Center of Intestinal and Colorectal Diseases,Hubei Key Laboratory of Intestinal and Colorectal Diseases,Hubei,Wuhan 430071,China

Received:2022-12-26 Accepted:2022-12-26 Online:2023-10-20 Published:2023-10-20

摘要/Abstract

摘要： 目的探讨LIM激酶1(LIMK1)在人肝癌细胞系Huh7中的表达及其对Huh7细胞的增殖、迁移及促进血管形成能力的影响。方法检测人正常肝细胞系 MIHA和人肝癌细胞系 Huh7 中LIMK1的 mRNA 和蛋白水平，siRNA转染Huh7细胞后通过CCK-8实验、克隆形成实验、划痕实验、Transwell实验和内皮细胞管形成实验探究对肝癌细胞增殖、迁移及血管形成能力的影响。结果 LIMK1在Huh7细胞中表达升高；敲低LIMK1后Huh7细胞的增殖及迁移能力显著下降，人脐静脉内皮细胞(HUVECs)体外血管形成能力减弱。结论 LIMK1在Huh7细胞中高表达，敲低LIMK1可抑制Huh7细胞的增殖、迁移及血管形成能力。

关键词: LIM激酶1, 肝细胞癌, 血管形成, 增殖, 迁移

Abstract: Objective To explore the expression of LIM kinase 1(LIMK1) in human hepatocellular carcinoma cell line Huh7 and its effects on the proliferation,migration and ability to promote angiogenesis of Huh7 cells. Methods The expression of LIMK1 mRNA and protein in human normal hepatocyte line MIHA and human hepatoma cell line Huh7 was detected.After targeted siRNA transfection of Huh7 cells,the effects on the proliferation,migration and angiogenesis ability of hepatocellular carcinoma cells were investigated by CCK-8 assay,clone formation assay,wound healing assay,Transwell assay and endothelial cell tube formation assay. Results LIMK1 was highly expressed in Huh7 cells.The proliferation and migration ability of Huh7 cells were significantly decreased after knocking down the expression level of LIMK1.Human umbilical vein endothelial cells(HUVECs) have reduced in vitro angiogenic capacity. Conclusion LIMK1 was highly expressed in Huh7 cells,and knockdown of LIMK1 inhibited the proliferation,migration and ability to promote angiogenesis of Huh7 cells.

Key words: LIM domain kinase 1, hepatocellular carcinoma, angiogenesis, proliferation, migration

劳垚佳程洁林梦璐张海平奉佳辉牟娟丽黄凯胡凡林军. LIM激酶1对肝癌细胞增殖、迁移及血管形成能力的影响[J]. 临床外科杂志, 2023, 31(10): 920-924.

LAO Yaojia, CHENG Jie, LIN Menglu, ZHANG Haiping, FENG Jiahui, MOU Juanli, HUANG Kai, HU Fan, LIN Jun. Effect of LIM kinase 1 on the proliferation,migration and ability to promote angiogenesis of hepatocellular carcinoma cells[J]. JOURNAL OF CLINICAL SURGERY, 2023, 31(10): 920-924.

参考文献

相关文章 15

[1]	郭晓丹马振禹王晨静韩天云张飞燕. LncRNA MIR205HG靶向miR-299-3p调控肺癌细胞增殖、凋亡的机制研究[J]. 临床外科杂志, 2023, 31(9): 838-842.
[2]	马立东刘晓蕾赵飞. 青蒿素通过TLR4/NF-κB信号通路调节胃癌细胞的生长和炎症因子分泌的机制研究[J]. 临床外科杂志, 2023, 31(8): 738-741.
[3]	曾强张宇陈辉石珂周丽. 富含亮氨酸的α2糖蛋白1通过RUNX1/OPN信号调节非小细胞肺癌细胞的增殖、迁移和侵袭 [J]. 临床外科杂志, 2023, 31(6): 562-567.
[4]	慕喜喜李静张玄高小鹏刘保荣钟岳. miR-3619-5p、LINC00857在原发性肝细胞癌血清中表达水平及其临床诊断价值分析[J]. 临床外科杂志, 2023, 31(6): 572-575.
[5]	李光泽张济林昌俭王垚柯俞慎林. 高迁移率蛋白1/Toll样受体4信号通路与下肢动脉硬化闭塞症介入治疗后复发的相关性分析[J]. 临床外科杂志, 2023, 31(6): 593-596.
[6]	刘金玮巴林超张劲夫范正军. 低氧脂滴诱导相关蛋白在肝细胞癌中的表达情况及临床意义[J]. 临床外科杂志, 2023, 31(5): 447-452.
[7]	张天翼高杨马尧任明智王孝彬马骏. LINC01197调控Notch通路促进肺腺癌细胞增殖和侵袭[J]. 临床外科杂志, 2023, 31(4): 335-339.
[8]	熊坚威武国赵开烽英保. 肝动脉化疗栓塞联合卡瑞利珠单抗成功转化不可切除肝癌一例[J]. 临床外科杂志, 2023, 31(2): 136-137.
[9]	林丽燕董佳肖伟进彭然吴方君马辉力超刘景丰. 基于全偏振成像和机器学习的肝细胞癌与肝内胆管癌鉴别诊断研究[J]. 临床外科杂志, 2023, 31(2): 164-167.
[10]	武烨晔王葵. 肝细胞癌的术后辅助治疗现状及展望[J]. 临床外科杂志, 2023, 31(10): 907-910.
[11]	徐剑军顾劲扬. 新辅助免疫治疗在肝癌肝移植中的研究进展[J]. 临床外科杂志, 2023, 31(10): 911-915.
[12]	杜佳家黄志勇. 肝细胞癌免疫治疗疗效预测的研究进展[J]. 临床外科杂志, 2023, 31(10): 990-993.
[13]	莫波王佩童宜欣何志军梁俊郝志楠. 膜解剖技术在胃癌根治术中应用的效果分析[J]. 临床外科杂志, 2022, 30(9): 835-838.
[14]	刘凯李勃吴向天明怀德. 二甲双胍通过LncRNA-H19抑制非小细胞肺癌A549迁移及侵袭的机制研究[J]. 临床外科杂志, 2022, 30(8): 719-721.
[15]	李新盖慧荣宋蕾张忠法. 长链非编码RNA SRGAP3-AS2异常表达通过Janus激酶信号调节非小细胞肺癌侵袭和迁移[J]. 临床外科杂志, 2022, 30(8): 727-730.

Metrics

Viewed

Full text

Abstract

Cited

Shared

Discussed

LIM激酶1对肝癌细胞增殖、迁移及血管形成能力的影响

Effect of LIM kinase 1 on the proliferation,migration and ability to promote angiogenesis of hepatocellular carcinoma cells

PDF (PC)

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

Metrics

本文评价

推荐阅读 10