关键词: 结直肠癌, 微小RNA-370-3p, Toll样受体4

Abstract: Objective To investigate the expression and clinical significance of microRNA(miR)-370-3p and Toll-like receptor 4(TLR4) in colorectal cancer.Methods The cancer tissues were collected from 146 patients with colorectal cancer in the Anorectal Surgery of our hospital.qRT-PCR was used to detect the expression levels of miR-370-3p and TLR4 mRNA in colorectal cancer tissues,immunohistochemical method was used to detect the expression level of TLR4 protein;the clinicopathological data of 146 patients were collected and and the patients were followed up for 3 years after operation,the relationship between the expression levels of miR-370-3p,TLR4 and clinicopathological characteristics and prognosis was analyzed;Pearson method was used to analyze the correlation between miR-370-3p and TLR4.ResultsThe expression level of miR-370-3p in colorectal cancer tissues(1.20±0.23) was lower than that in adjacent tissues(1.94±0.32)(P＜0.05);the level of TLR4 mRNA in cancer tissues(2.46±0.56) was significantly higher than that in adjacent tissues(1.39±0.25)(P＜0.05);the high expression rate of TLR4 protein in cancer tissues 40.41%(59/146) was higher than 13.01%(19/146) in adjacent tissues(P＜0.05);the expression levels of miR-370-3p and TLR4 in cancer tissues were related to lymph node metastasis,TNM staging,and tumor differentiation(P＜0.05);miR-370-3p was negatively correlated with TLR4 in colorectal cancer tissue(r=-0.760,P=0.000);miR-370-3p,lymph node metastasis,TLR4,TNM staging,and degree of differentiation were related factors affecting the prognosis of patients(P＜0.05);when miR-370-3p was highly expressed and TLR4 was lowly expressed,the cumulative survival rate of patients was higher.Conclusion miR-370-3p and TLR4 are low and high expression in colorectal cancer,respectively,and they are negatively correlated.Their expression level is related to colorectal cancer stage,invasion,differentiation and poor prognosis.

Key words: colorectal cancer, microRNA-370-3p, Toll-like receptor 4