Abstract: Objective To analyze the relationship of thyroid hormone responsive spot 14 protein (THRSP),plasminogen activator urokinase (PLAU),E26 transformation-specific variant 4 (ETV4) and cervical lymph node metastasis and prognostic of papillary thyroid carcinoma (PTC).Methods Totally 108 PTC patients who received treatment in our hospital between July 2017 and June 2021 were gathered.Immunohistochemistry was used to detect the expression of THRSP,PLAU,and ETV4 in cancer tissues and adjacent tissues.Kaplan-Meier method was used to analyze the relationship between THRSP,PLAU,and ETV4 with the survival rate of PTC.Cox regression was used to analyze the influencing factors of poor prognosis in PTC.Results The positive expression rates of THRSP,PLAU,and ETV4 in PTC cancer tissues (60.19%,62.04%,56.48%) were manifestly higher than those in adjacent tissues (37.04%,39.81%,37.96%) (P<0.05).PTC patients with lymph node metastasis had manifestly higher positive expression rates of THRSP,PLAU,and ETV4 (80.77%,80.77%,76.92%) than non lymph node metastasis (53.66%,56.10%,50.00%) (P<0.05).The results of Kaplan-Meier analysis showed that the 3-year progression free survival rate of the THRSP,PLAU,and ETV4 positive expression groups (60.00%,59.70%,60.66%) was manifestly lower than that of the negative expression groups (88.37%,90.24%,85.11%) (P<0.05).The good prognosis group had manifestly lower rates of lymph node metastasis,THRSP,PLAU,and ETV4 expression (12.99%,50.65%,51.95%,48.05%) than the poor prognosis group (51.61%,83.87%,87.10%,77.42%) (P<0.05).Cox analysis found that lymph node metastasis (HR=3.736,95%CI=1.669-8.361),THRSP,PLAU and ETV4 were factors affecting poor prognosis in PTC (P<0.05).Conclusion THRSP,PLAU,and ETV4 are positively expressed in PTC cancer tissues.The 3-year survival rate of PTC patients with positive THRSP (HR=3.218,95%CI=1.366-7.578),PLAU (HR=3.567,95%CI=1.548-8.221) and ETV4 (HR=4.103,95%CI=1.348-12.491) expression is relatively low.These three factors are associated with cervical lymph node metastasis and prognosis.

Key words: thyroid hormone responsive spot 14 protein; plasminogen activator urokinase; E26 transformation-specific variant 4; papillary thyroid carcinoma; lymph node metastasis; prognosis