Abstract: Objective  To investigate the expression of RNFT2,MFAP2,and AF4/FMR2 family member 3 (AFF3) in gastric cancer(GC) tissues,and to analyze their correlation with tumor pathological features and prognosis.Methods GC tissue specimens and adjacent tissue specimens were collected from 98 GC patients who underwent surgery in our hospital from May 2018 to January 2020,clinical data were also collected.Immunohistochemical methods were applied to detect the expression of RNFT2,MFAP2,and AFF3;Kaplan-Meier method was applied to analyze the relationship between the expression of RNFT2,MFAP2,and AFF3 and the prognosis of GC patients;multivariate Cox regression was applied to analyze the influencing factors of prognosis in GC patients.Results  Compared with adjacent tissues,the positive rates of RNFT2 and MFAP2 proteins in GC tissue were obviously higher（75.51% vs. 29.59%,70.41% vs.19.39%),while the positive rate of AFF3 protein was obviously lower (26.53% vs. 68.37%,P<0.05).The expression of RNFT2,MFAP2,and AFF3 proteins was obviously correlated with TNM staging and differentiation degree (P<0.05);Kaplan-Meier method showed that the 3 year survival rates of RNFT2 and MFAP2 positive expression patients were lower than those of RNFT2 and MFAP2 negative expression patients,respectively,the 3year survival rate of AFF3 positive expression patients was higher than that of AFF3 negative expression patients (P<0.05).Multivariate Cox regression analysis showed that RNFT2,MFAP2,AFF3,TNM staging,and differentiation degree were prognostic factors for GC patients.Conclusion RNFT2 and MFAP2 are obviously upregulated in GC tissue,while AFF3 is obviously downregulated,which is closely related to clinical pathological features (such as TNM staging and differentiation) and prognosis,and has important value for the prognosis and survival of GC patients.

Key words: ring finger transmembrane protein 2, microfibrillar associated protein 2, AF4/FMR2 family member 3, gastric cancer, pathological features, prognosis