股骨颈骨折病人血清微小RNA-21-5p、微小RNA-1224-5p、微小RNA-181a-5p表达与术后愈合延迟的相关性

doi:10.3969/j.issn.1005-6483.20241508

临床外科杂志 ›› 2025, Vol. 33 ›› Issue (10): 1105-1108.doi: 10.3969/j.issn.1005-6483.20241508

股骨颈骨折病人血清微小RNA-21-5p、微小RNA-1224-5p、微小RNA-181a-5p表达与术后愈合延迟的相关性

郭建郭月超王哲安文张玉舰

066000 河北秦皇岛，秦皇岛市第一医院骨科(郭建、郭月超、安文、张玉舰)，手术室(王哲)

收稿日期:2024-09-13 出版日期:2025-11-11 发布日期:2025-11-11
通讯作者: 张玉舰，Email:qhd-zyj@163.com
基金资助:
河北省医学科学课题研究计划(20240650)

The correlation between serum miR-21-5p,miR-1224-5p,miR-181a-5p expression with postoperative delayed healing in patients with femoral neck fracture

GUO Jian^*,GUO Yuechao,WANG Zhe,AN Wen,ZHANG Yujian

^*Department of Orthopedics,the First Hospital of Qinhuangdao,Hebei,Qinhuangdao 066000,China

Received:2024-09-13 Online:2025-11-11 Published:2025-11-11

摘要/Abstract

摘要： 目的探讨股骨颈骨折(FNF)病人血清微小RNA-21-5p(miR-21-5p)、微小RNA-1224-5p(miR-1224-5p)、微小RNA-181a-5p(miR-181a-5p)表达与术后愈合延迟的相关性。方法 2021年10月~2024年4月收治的FNF病人371例，根据术后4个月骨折是否愈合将病人分为研究组(延迟愈合，68例)和对照组(正常愈合，303例)。采用实时荧光定量PCR(qRT-PCR)检测血清miR-21-5p、miR-1224-5p、miR-181a-5p的表达水平；多因素Logistic回归分析FNF病人术后愈合延迟的影响因素；ROC曲线分析血清miR-21-5p、miR-1224-5p、miR-181a-5p对FNF病人术后愈合延迟的预测价值。结果研究组血清miR-21-5p、miR-1224-5p、miR-181a-5p水平均低于对照组(P<0.05)；多因素Logistic回归分析结果显示，年龄≥60岁、糖尿病、吸烟是FNF病人术后愈合延迟的危险因素(P＜0.05)，miR-21-5p、miR-1224-5p、miR-181a-5p是FNF病人术后愈合延迟的保护因素(P＜0.05)。ROC曲线分析显示，miR-21-5p、miR-1224-5p、miR-181a-5p预测FNF病人术后愈合延迟的AUC值分别为0.845、0.809、0.797，三者联合预测时，AUC提升为0.933，三者联合检测效果优于任一指标单独检测(Z_联合vs. miR-21-5p=3.444、Z_联合vs. miR-1224-5p=4.401、Z_联合vs. miR-181a-5p=4.279，P＜0.05)。结论术后愈合延迟的FNF病人血清miR-21-5p、miR-1224-5p、miR-181a-5p水平降低，三者联合检测可能对FNF病人术后愈合延迟具有一定预测价值。

关键词: 股骨颈骨折, miR-21-5p, miR-1224-5p, miR-181a-5p, 愈合延迟

Abstract: Objective To investigate the correlation between the expression of serum microRNA-21-5p (miR-21-5p),microRNA-1224-5p (miR-1224-5p),and microRNA-181a-5p (miR-181a-5p) with postoperative delayed healing in patients with femoral neck fracture (FNF).Methods From October 2021 to April 2024,371 patients with FNF admitted to our hospital were included as subjects.The patients were assigned into a study group (delayed healing,68 cases) and a control group (normal healing,303 cases) based on whether the fracture ends healed 4 months after surgery.Real-time fluorescence quantitative PCR (qRT-PCR) was applied to detect the expression levels of serum miR-21-5p,miR-1224-5p,and miR-181a-5p.Multivariate Logistic regression was applied to analyze the influencing factors of postoperative healing delay in FNF patients.ROC curve was applied to analyze the predictive value of serum miR-21-5p,miR-1224-5p,and miR-181a-5p for postoperative healing delay in FNF patients.Results The levels of serum miR-21-5p,miR-1224-5p,and miR-181a-5p in the study group were lower than those in the control group (P<0.05).Multivariate Logistic analysis showed that age ≥60 years old,diabetes,and smoking were risk factors for postoperative delayed healing in FNF patients (P<0.05),and miR-21-5p,miR-1224-5p and miR-181a-5p were protective factors for postoperative delayed healing in FNF patients (P<0.05).ROC curve showed that the AUC values of miR-21-5p,miR-1224-5p,and miR-181a-5p for predicting postoperative healing delay in FNF patients were 0.845,0.809,and 0.797,respectively.When the three were combined for prediction,the AUC increased to 0.933.The combined detection effect of the three indicators was better than that of any individual indicator (Z_combination vs. miR-21-5p=3.444,Z_combination vs. miR-1224-5p=4.401,Z_combination vs. miR-181a-5p=4.279,P<0.05).Conclusion The levels of serum miR-21-5p,miR-1224-5p,and miR-181a-5p are reduced in FNF patients with postoperative delayed healing.The combined detection of the three may have a certain predictive value for postoperative delayed healing in FNF patients.

Key words: femoral neck fracture, miR-21-5p, miR-1224-5p, miR-181a-5p, delayed healing

郭建郭月超王哲安文张玉舰. 股骨颈骨折病人血清微小RNA-21-5p、微小RNA-1224-5p、微小RNA-181a-5p表达与术后愈合延迟的相关性[J]. 临床外科杂志, 2025, 33(10): 1105-1108.

GUO Jian, GUO Yuechao, WANG Zhe, AN Wen, ZHANG Yujian. The correlation between serum miR-21-5p,miR-1224-5p,miR-181a-5p expression with postoperative delayed healing in patients with femoral neck fracture[J]. JOURNAL OF CLINICAL SURGERY, 2025, 33(10): 1105-1108.

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股骨颈骨折病人血清微小RNA-21-5p、微小RNA-1224-5p、微小RNA-181a-5p表达与术后愈合延迟的相关性

The correlation between serum miR-21-5p,miR-1224-5p,miR-181a-5p expression with postoperative delayed healing in patients with femoral neck fracture

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