关键词: 舒芬太尼； AMPK-SIRT1-PGC-1α信号通路； 甲状腺癌； 增殖； 迁移； 侵袭； 凋亡

Abstract: Objective To investigate the effects of sufentanil on proliferation,invasion,migration,and apoptosis of thyroid cancer cells,and analyze whether its mechanism is related to the AMP-activated protein kinase(AMPK) -silent information regulator 1(SIRT1) -peroxisome proliferator activator receptor gamma coactivator 1α(PGC-1α) signaling pathway.Methods The human thyroid cancer cell line(TPC-1) was separated into a control group,L,M,H-sufentanil groups,and H-sufentanil+AICAR group.TPC-1 cell activity,apoptosis,invasion,migration,and expression of proteins were detected using CCK-8 assay,flow cytometry,Transwell assay,scratch assay,and Western blot,respectively.〖WTHZ〗ResultsCompared with the control group,the absorbance value,invasion number,scratch healing rate,cleaved-Caspase-3,programmed cell death 1 ligand 1,B lymphoblastoma-2,p-AMPK/AMPK,SIRT1,and PGC-1α protein levels in L,M,and H-sufentanil groups were reduced;the apoptosis rate and Bax protein level were increased(P＜0.05).AICAR treatment reversed the effect of H-sufentanil on the above indicators(P＜0.05).Conclusion Sufentanil may inhibit the AMPK-SIRT1-PGC-1α signaling pathway,thereby inhibiting the proliferation and invasion migration of thyroid cancer cells,and promoting cell apoptosis.

Key words: sufentanil;AMPK-SIRT1-PGC-1α signaling pathway;thyroid cancer;proliferation;migration;invasion;apoptosis