关键词: LINC01197, Notch, 肺腺癌, 增殖, 侵袭

Abstract: Objective  To reveal the expression and underlying mechanism of LINC01197 in lung adenocarcinoma(LUAD).  Methods   The expression of LINC01197 was detected by real-time fluorescence quantitative PCR(qRT-PCR),and the expression of Notch signaling molecules(Notch1 and Hes1) were detected by Western blot.Cell proliferation and invasion ability were analyzed by MTT,colony formation and Transwell assays.The effect of LINC01197 on tumor growth was examined in a xenograft tumor assay.  Results  LINC01197 was down-regulated in LUAD,and the protein levels of Notch1 and Hes1 were both up-regulated(P<0.05).Overexpression of LINC01197 inhibited LUAD cell proliferation and invasion,while knockdown of LINC01197 had the opposite effect(P<0.05).Interestingly,LINC01197-induced changes in cell behavior could be reversed by Notch1 modulators(P<0.05).Furthermore,LINC01197 overexpression inhibited tumor growth and Notch signaling in vivo(P<0.05).  Conclusion  LINC01197 can exhibit anti-proliferation and anti-invasion effects in LUAD by inhibiting Notch signaling pathway.

Key words: LINC01197, Notch, lung adenocarcinoma, proliferation, invasion