关键词: 急性胰腺炎； 微小RNA-217-5p； 微小RNA-325-3p

Abstract: Objective To investigate the relationship between serum microRNA-217-5p (miR-217-5p) and microRNA-325-3p (miR-325-3p) expression with the occurrence and development of acute pancreatitis (AP).Methods From February 2021 to February 2024,127 patients with acute pancreatitis (AP) treated in our hospital were included as the AP group.AP was further separated into mild acute pancreatitis (MAP) group (n=44),moderate to severe acute pancreatitis (MSAP) group (n=42),and severe acute pancreatitis (SAP) group (n=41) according to the severity of the disease.During the same period,127 healthy volunteers who underwent physical examination in our hospital were included as the control group.qRT-PCR method was applied to detect the relative expression levels of serum miR-217-5p and miR-325-3p.Multivariate Logistic regression was applied to analyze the influencing factors of SAP.ROC curves were applied to investigate the diagnostic value of serum miR-217-5p and miR-325-3p for SAP.Results The relative expression level of serum miR-217-5p in the AP group was 1.52±0.16,which was significantly higher than that in the control group（1.02±0.13）.The relative expression level of miR-325-3p was 0.71±0.08,which was significantly lower than that in the control group（1.01±0.12） (P<0.05).The relative expression levels of miR-217-5p in the MAP group,MSAP group,and SAP group were 1.34±0.15,1.55±0.17 and 1.67±0.21,respectively,and the levels of C-reactive protein (CRP) were （27.07±6.31）mg/L,（35.37±7.58）mg/L and（51.04±8.14）mg/L,respectively,and the content of procalcitonin (PCT) was （0.16±0.03）ng/ml,（0.19±0.04）ng/ml and （0.26±0.04）ng/ml,respectively,which were significantly increased (P<0.05).MiR-217-5p was an independent risk factor affecting SAP (P<0.05).MiR-325-3p was an independent protective factor affecting SAP (P<0.05).The AUC of serum miR-217-5p and miR-325-3p for diagnosing SAP alone was 0.831 and 0.861,respectively.The AUC of their combined diagnosis was 0.941,which was better than the AUC of their combined diagnosis (Z_{miR-217-5p combination}=3.003,Z_{miR-325-3p combination}=2.725,both P<0.05).Conclusion Serum miR-217-5p expression is upregulated and miR-325-3p expression is downregulated in the serum of SAP patients,and they are associated with the occurrence and development of AP.Abnormal expressions of both are factors affecting SAP,and the combination of the two has higher diagnostic value for SAP.

Key words: acute pancreatitis; microRNA-217-5p; microRNA-325-3p