关键词: 膀胱尿路上皮癌, 微小RNA-34a-5p, 细胞分裂周期蛋白20

Abstract: Objective To investigate the expression of cell division cycle protein 20(CDC20) and microRNA-34a-5p(miR-34a-5p) in bladder urothelial carcinoma(BUC) and their correlation with clinicopathology.
Methods 80 patients with BUC in our hospital were selected. All of them received surgical treatment, the cancer tissue (BUC group) and the corresponding adjacent tissue (control group, 2.5 cm away from the cancer edge) were taken during the operation.The relationship between expression levels of CDC20 and miR-34a-5p and clinicopathological features were analyzed.Analyze the risk factors affecting the prognosis of patients with BUC.
Results The expression level of CDC20 mRNA in BUC group was higher than that in control group,and the expression level of miR-34a-5p in BUC group was lower than that in control group(P<0.05).The positive rate of CDC20 in BUC group was higher than that in adjacent normal tissue(P<0.05).The expression of CDC20 and miR-34a-5p in patients with BUC was closely related to histological grade,lymph node metastasis,tissue invasion and recurrence.The 3-year cumulative survival rate of patients with positive expression of CDC20 was lower than that of patients with negative expression of CDC20;the 3-year cumulative survival rate of patients with high expression of miR-34a-5p was higher than that of patients with low expression of miR-34a-5p.Multivariate COX analysis showed that high CDC20 level and low miR-34a-5p level were risk factors for poor prognosis of BUC(P<0.05).
Conclusion The expression of CDC20 is high and the expression of miR-34a-5p is low in BUC,they are closely related to histological grade,lymph node metastasis,tissue invasion,recurrence and poor prognosis,and may be used as potential diagnostic markers.

Key words: bladder urothelial cancer, microRNA-34a-5p, cell division cycle protein 20