关键词: 肾细胞癌, 动物模型, 人源性肿瘤组织异种移植物, 精准医学, 个性化治疗

Abstract: Objective Establishment of human renal cell carcinoma in NOD-SCID mice subcutaneous xenograft model and subcapsular of renal transplantation tumor model(for example,orthotopic transplantation tumor model),its biological characteristics were analyzed.Methods Tissue specimens were obtained from patients with clinically preoperative diagnosis of renal cell carcinoma.And then sheared the tissue to subcutaneous and orthotopic inoculated to NOD-SCID mice.To prepare xenograft models of primary and passage xenograft models.It was obseved of tumor xenograft model of the incubation period,the rate of tumor formation,tumor volume,tumor invasion and metastasis.Results PDX tumor patients,5 males and 2 females,which accounted for 85.7%（6 cases) of renal clear cell carcinoma,renal parenchyma of pleomorphic epithelioid cell tumor accounted for 14.3%(1 case).One patient was with renal vein tumor thrombus.NOD/SCID mice transplanted tumor latent period was 63.83 days,the rate of tumor formation was about 42.86%,the growth rate of transplanted tumor was different,and the volume was different,for:6.426-197.856 mm-3.It was found that the transplanted tumor was similar to the human renal cell carcinoma from pathological changes of transplanted tumor.Conclusion Compared with orthotopic transplantation,subcutaneous transplantation tumor model was easy to operate,easy to evaluate,measure and observe the tumor.But orthotopic transplantation of the best advantages of the biological behavior of renal cell carcinoma which could better simulate the occurrence and development of the tumor.The transplanted tumor of mice was similar to the structure of human renal cell carcinoma,which could be used as a powerful tool for preclinical study of renal cell carcinoma.

Key words: renal cell carcinoma；animal model；patient derived xenografts；precision medicine, personalized therapy